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lamp2  (Cell Signaling Technology Inc)
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Cell Signaling Technology Inc lamp2
Fig. 2. TSA inhibits MI-induced impairment of autophagosome clearance in vivo. A, Immunoblots and (B) quantitative analysis of LC3, P62, beclin 1, <t>LAMP2,</t> Bcl2 and Bax protein expression in mice myocardium from the different groups (n=3). C, Representative immunofluorescence and quantitative analysis of P62 and the cardiac fibroblast marker vimentin (n=3). *P<0.05 vs the indicated group. MI, myocardial infarction; TSA, trichostatin A.
Lamp2, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Fig. 2. TSA inhibits MI-induced impairment of autophagosome clearance in vivo. A, Immunoblots and (B) quantitative analysis of LC3, P62, beclin 1, LAMP2, Bcl2 and Bax protein expression in mice myocardium from the different groups (n=3). C, Representative immunofluorescence and quantitative analysis of P62 and the cardiac fibroblast marker vimentin (n=3). *P<0.05 vs the indicated group. MI, myocardial infarction; TSA, trichostatin A.

Journal: Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology

Article Title: Inhibition of Histone Deacetylases Prevents Cardiac Remodeling After Myocardial Infarction by Restoring Autophagosome Processing in Cardiac Fibroblasts.

doi: 10.1159/000493672

Figure Lengend Snippet: Fig. 2. TSA inhibits MI-induced impairment of autophagosome clearance in vivo. A, Immunoblots and (B) quantitative analysis of LC3, P62, beclin 1, LAMP2, Bcl2 and Bax protein expression in mice myocardium from the different groups (n=3). C, Representative immunofluorescence and quantitative analysis of P62 and the cardiac fibroblast marker vimentin (n=3). *P<0.05 vs the indicated group. MI, myocardial infarction; TSA, trichostatin A.

Article Snippet: Proteins were separated by SDS-PAGE, transferred to nitrocellulose, and immunoblotted with antibodies against LC3 (Sigma-Aldrich, L8918), p62 (Cell Signaling Technology, #5114), GAPDH (Cell Signaling Technology, #5174), LAMP2 (Cell Signaling Technology, #49067), Bcl2 (Cell Signaling Technology, #2872), and Bax (Cell Signaling Technology, #14796).

Techniques: In Vivo, Western Blot, Expressing, Immunofluorescence, Marker

Fig. 5. Restoration of autophagic flux is responsible for the protective effects of TSA against hypoxia-induced cell death. A, Immunoblotting analysis of LAMP2 protein levels. B, Mitochondrial membrane potential (B) and cell death (C) after hypoxia with or without CQ and adenovirus encoding short hairpin RNA of LAMP2a. *P<0.05 vs the indicated group (n=3). TMRE, tetramethylrhodamine ethyl ester; TSA, trichostatin A; CQ, chloroquine.

Journal: Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology

Article Title: Inhibition of Histone Deacetylases Prevents Cardiac Remodeling After Myocardial Infarction by Restoring Autophagosome Processing in Cardiac Fibroblasts.

doi: 10.1159/000493672

Figure Lengend Snippet: Fig. 5. Restoration of autophagic flux is responsible for the protective effects of TSA against hypoxia-induced cell death. A, Immunoblotting analysis of LAMP2 protein levels. B, Mitochondrial membrane potential (B) and cell death (C) after hypoxia with or without CQ and adenovirus encoding short hairpin RNA of LAMP2a. *P<0.05 vs the indicated group (n=3). TMRE, tetramethylrhodamine ethyl ester; TSA, trichostatin A; CQ, chloroquine.

Article Snippet: Proteins were separated by SDS-PAGE, transferred to nitrocellulose, and immunoblotted with antibodies against LC3 (Sigma-Aldrich, L8918), p62 (Cell Signaling Technology, #5114), GAPDH (Cell Signaling Technology, #5174), LAMP2 (Cell Signaling Technology, #49067), Bcl2 (Cell Signaling Technology, #2872), and Bax (Cell Signaling Technology, #14796).

Techniques: Western Blot, Membrane, shRNA